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February 23, 2014


Attractability of Probiotic-Coated Shrimp Feed


Many shrimp farmers top-dress their feed with probiotics to deliver favorable microorganisms to the gastrointestinal tract of shrimp.  One of these probiotics is INVE’s Sanolife PRO-2, a mixture of selected Bacillus strains selected for their ability to inhibit pathogens, be metabolically active in the shrimp’s gut and in the shrimp pond and improve digestibility.  Typically, farmers coat the probiotics on commercial feed using either water or fish oil as binder.


The aim of this short experiment was to evaluate the attractability of shrimp feed that had been coated with Sanolife PRO-2.  To avoid interference with the binder, the probiotics were coated using seawater.


The experiment took place at INVE’s Shrimp Culture Research and Development facilities, in Chonburi, Thailand.  Penaeus vannamei juveniles (six to eight grams) were stocked in 175-liter fiberglass tanks at ten animals per tank, and starved for a period of 48 hours prior to the onset of the experiment.  With four replicates per treatment, two feeds were evaluated:


• A control feed (Charoen Pokphand’s No. 5-9505) mixed with 100 milliliters of seawater per kilogram of feed and left to dry.


• A treatment feed (Charoen Pokphand’s No. 5-9505) mixed with 100 milliliters seawater and five grams of Sanolife PRO-2 per kilogram of feed and left to dry.


Each tank contained two feeding trays: one for the control feed and one for the treatment feed.  One hour before feeding, the aeration was stopped.  Immediately after the feed test, aeration was restored.


Shrimp were fed for one hour in the morning.  Subsequently, all feeding trays were removed from the tanks and the amount of pellets remaining on each tray was counted.  In the evening, small amounts of feed were given.  All feed present on the trays overnight had to be consumed so that in the morning the animals would be hungry and a new palatability test could be done. The number of pellets consumed by the animals each morning was counted for seven days.


Tanks were siphoned every morning, before feeding. Dead animals were removed and replaced by animals that had been cultured in a separate tank.


On average, the number of ingested feed pellets, after one hour, was 1.9, 1.5, 2.2, and 2.6 times higher when the feed was coated with INVE’s probiotic!


Information: INVE Technologies, Hoogveld 93, 9200 Dendermond, Belgium (phone +32-0-52-40-95-95, fax +32-0)-52-40-95-80, email, webpage


Source: AQUA Culture AsiaPacific (Editor/Publisher, Zuridah Merican, email  Attractability of Probiotic-Coated Shrimp Feed.    Phuthongphan Rattayaporn (, Srianek Patipon, Roeland Wouters (, Geert Rombaut ( and Olivier Decamp (  Volume 10, Number 1, Page 38, January/February 2014.

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