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May 10, 2013


Vibrio parahaemolyticus, Phages and Brine Shrimp


Shrimp News: I don’t pretend to understand all this stuff about Vibrio parahaemolyticus and phages, but since this study mentions both of them in conjunction with brine shrimp, I thought that those in the scientific community might glean something from it that will lead to a better understanding of early mortality syndrome in shrimp.


Study’s Highlights


• A challenge model was developed to evaluate the control of vibriosis in Artemia.

• Phage therapy was evaluated to control the mortality induced by Vibrio parahaemolyticus.

• The effect of delayed phage therapy were compared with oxytetracycline treatment.

• Efficacy of phage therapy is compromised by the degree of advance of the infection.


Study’s Abstract


“Phage therapy is an alternative to control bacterial pathogens in aquaculture.  The extensive use is apparently feasible, however it is necessary to know its potential limitations arising from the particular characteristics of the aquatic environment in terms of the dynamics of bacterial infections, in particular because under some circumstances, the direct release of phages is the unique alternative to apply the treatment to the cultures.  In the present study the efficacy of phage therapy was evaluated under gnotobiotic conditions during the induced vibriosis of Artemia franciscana.  Axenic brine shrimp nauplii were infected with Vibrio parahaemolyticus and treated with phage therapy, the effect of the reduction in the phage dose and the effect of delay treatment were evaluated.  The vibriosis was successfully prevented by phage therapy; a single dosage of vpms1 phage was effective to eliminate the adverse effects of V. parahaemolyticus in brine shrimp (P <0.001) and their efficacy was not affected by the reduction in the dosage, even at a multiplicity of infection of 0.45.  However, its beneficial effects were compromised during the infection progress; when the application of phages was delayed, phage therapy was ineffective to control the mortality induced by V. parahaemolyticus. In conclusion, under the evaluated conditions the phage therapy was effective to prevent vibriosis in brine shrimp, however, in advanced infections their ability to control the vibriosis is limited.”


Source: Aquaculture.  Efficacy of Phage Therapy to Prevent Mortality During the Vibriosis of Brine Shrimp.  S.F. Martínez-Díaz ( and A. Hipólito-Morales (Microbiology and Molecular Biology Laboratory, Interdisciplinary Center of Marine Science, CICIMAR-IPN, Playa el Conchalito sn, La Paz, Baja California Sur, CP 23090, Mexico)  Volume 400–401, Pages 120–124, June 20, 2013.


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