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January 18, 2014


Using Phage Cocktails to Fight EMS


From Abstract: Phage therapy can be an eco-friendly alternative to prevent and control pathogenic bacteria in aquaculture.  Although phage bacterial resistance is well documented in the scientific literature, the use of phage cocktails can overcome this drawback.  The aim of this study was to evaluate the efficiency of cocktails of two and three phages of Vibrio parahaemolyticus (VP-1, VP-2 and VP-3) to control Vibrios in aquaculture.  All three phages were effective against V. parahaemolyticus; however, the VP-3 phage was the most efficient one (additional reduction of more 2 log when compared with the other two phages).  The use of cocktails with two and three phages was significantly more effective (reductions of 4 log after 2 hours) than the use of VP-1 and VP-2 phages alone (reductions of 0.8 log after 2 hours).  All phages remained viable for a long time (at least 5-7 months) in marine water.  The VP-3 phage presented a larger burst size and a shorter latent period than the other two phages.  Overall, the use of phage cocktails of two or three phages increased the efficiency of phage therapy against Vibrio (more efficient and faster bacterial inactivation) and delayed the development of resistance by the bacteria.  The use of Vibrio phages with high burst sizes and short lytic cycles also increased the efficiency of phage therapy.


Source: Aquaculture.  Efficiency of Phage Cocktails in the Inactivation of Vibrio in Aquaculture.  L. Mateus, L. Costa, Y.J. Silva, C. Pereira, A. Cunha and A. Almeida (, Departamento de Biologia and CESAM, Universidade de Aveiro, Campus Universitário de Santiago, Aveiro, Portugal, phone +351-234370350, fax + 351-234372587).  In Press.  Available Online January 10, 2014.

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