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May 16, 2014

Portugal

Phages and Lysozyme Against Vibrio parahaemolyticus

 

From Abstract: Phage therapy [using viruses to infect and kill bacteria] has gained increased attention as an alternative to antibiotics in order to control bacterial diseases and prevent the spreading of multidrug-resistant bacteria in aquaculture.  In this study, the addition of a lytic enzyme (lysozyme) during phage therapy was evaluated for the first time to control Vibrio infection.  Three phages of Vibrio parahaemolyticus (VP-1, VP-2 and VP-3) were used.  The results indicate that the combination of lysozyme and phage showed better activity in comparison to the activity of the phage alone.  VP-1 and VP-2 phages in the presence of lysozyme were more effective in reducing bacterial concentration (reductions of 4.0 log) than the VP-3 phage (which was the most efficient against Vibrio).  The application of phages along with lysozyme can be a very useful strategy to eliminate or reduce pathogenic bacteria in aquaculture, namely when less effective phages are available.

 

Source: Aquaculture. Effect of Lysozyme Addition on the Activity of Phages Against Vibrio Parahaemolyticus.  L. Mateus, L. Costa, Y.J. Silva, C. Pereira, A. Almeida (email aalmeida@ua.pt, Departamento de Biologia and CESAM, Universidade de Aveiro, Campus Universitário de Santiago Aveiro, Portugal).  In Press, Accepted Manuscript, Available Online May 9, 2014.

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