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January 7, 2014


PCR Detection of EMS, Update


On January 6, 2014, National Cheng Kung University (NCKU) announced its PCR-based method to detect early mortality syndrome (EMS) in shrimp, developed by Dr. Chu-Fang Lo, Dean of the College of Life Sciences at NCKU, in cooperation with Dr. Tim Flegel from Mahidol University in Thailand.


Dr. Lo also said, “We decided to release, free for public access, detailed information on the sequences and protocols....” She also noted that the bacteria causing AHPND was Vibrio parahaemolyticus (Vp), but with unique plasmids not present in non-pathogenic Vp.


Dr. Lo’s team used a whole-genome sequencing strategy to identify the plasmids as targets for detection, and they designed two pairs of primers (AP1 and AP2) for the PCR method“If shrimp has virulent Vp, the AP1 and AP2 primers will identify the Vp and give a positive reaction,” said Dr. Lo.


Information: Professor T.W. Flegel, Center of Excellence for Shrimp Molecular Biology and Biotechnology, Faculty of Science, Mahidol University, Rama VI Road, Bangkok 10400, Thailand, and the National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Thailand Science Park, Phaholyothin Road, Khlong Luang, Pathum Thani, 12120, Thailand.


Information Dr. Chu-Fang Lo, Dean of the College of Life Sciences, Center of Bioscience and Biotechnology, College of Bioscience and Biotechnology, National Cheng Kung University, Tainan, Taiwan and Department of Life Science, National Taiwan University, Taipei, Taiwan, Republic of China (phone 06-2757575, Ext.65500, email


Information: Ms. Udomrat Vatanakul, Manager, Public Relations Section at Thailand’s National Center for Genetic Engineering and Biotechnology (BIOTEC, phone 66-2-564-6700, extension 3324, fax 66-2-564-6572, email


Source: National Cheng Kung University’s Website.  NCKU-Thailand Research Team Releases a PCR-Based Method to Detect Early Mortality Syndrome (EMS) of Shrimp.  January 7, 2014,

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