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March 21, 2014

Thailand

Alternative to Eyestalk Ablation

 

In this study, the recombinant gonad-inhibiting hormone of Penaeus monodon was expressed (produced) in Pichia pastoris, a species of yeast widely used for protein expression using recombinant DNA techniques.  A single injection of anti-GIH mAb into wild previtellogenic P. monodon was able to induce ovarian maturation and spawning at a comparable rate to eyestalk ablation.  The results demonstrate the neutralization effect of anti-GIH mAb on vitellogenesis inhibitory action of GIH, and thus provide a potential alternative to induce ovarian maturation in P. monodon broodstock.

 

From Abstract: “Ovarian maturation in crustacean is controlled by an eyestalk neuropeptide called gonad-inhibiting hormone (GIH) that is presumed to inhibit vitellogenin synthesis.  In this study, a recombinant protein of GIH of Penaeus monodon (rPem-GIH) was expressed in a Pichia pastoris expression system and purified to homogeneity by reversed-phase SPE (C18).  The purified rPem-GIH significantly reduced vitellogenin mRNA level in primary tissue culture derived from previtellogenic ovary of P. monodon broodstock by 45.7% compared with the untreated group. This effect was similar to that of partially purified optic lobe extract, and demonstrated that the rPem-GIH possessed gonad-inhibiting activity.  A monoclonal antibody specific to Pem-GIH (GIH mAb) was able to neutralize the activity of GIH from the partially purified optic lobe extract to about 58 % by in vitro assay in primary tissue culture of the ovary.  Peptide mapping revealed that amino acids 16 to 20 (MYNKV) of the mature Pem-GIH may serve as an epitope of anti-GIH mAb.”

 

Source: Aquaculture.  Functional Characterization of Recombinant Gonad-Inhibiting Hormone (GIH) and Implication of Antibody Neutralization on Induction of Ovarian Maturation in Marine Shrimp.  Supattra Treerattrakool email (tsupattra@gmail.com, Institute of Molecular Biosciences, Mahidol University, Salaya Campus, Nakhon Pathom 73170, Thailand), Chanikarn Boonchuoy, Sittichai Urtgam, Sakol Panyima and Apinunt Udomkita.  In Press, Accepted Manuscript, Available Online March 20, 2014.

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