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April 5, 2014


Diversity in Isolates That Cause EMS


From Abstract: In this study, researchers describe the isolation and characterization of bacteria isolated from the hepatopancreas of shrimp collected from a farm in Thailand that experienced early mortality syndrome (EMS).  Four independent bacterial isolates were identified as Vibrio parahaemolyticus by BLAST analysis and by gene-specific marker detection of a lecithin dependent hemolysin considered being specific for the species.  Immersion challenges with three of these and a reference isolate obtained from China in 2010 caused very high mortality accompanied by characteristic histopathology in the hepatopancreas.  Tests with one of these isolates revealed that the rate of mortality was dose dependent.  Using the same challenge protocol, another isolate also caused high mortality, but it was not accompanied by EMS histopathology.  Instead, it caused a different histopathology of the hepatopancreas, including collapsed epithelia and unique vacuolization of embryonic cells.  These results revealed the possibility of diversity in isolates of V. parahaemolyticus that may cause EMS.


Source: Aquaculture.  Variation in Vibrio Parahaemolyticus Isolates from a Single Thai Shrimp Farm Experiencing an Outbreak of Acute Hepatopancreatic Necrosis Disease (AHPND).  Jyoti Joshi, Jiraporn Srisala, Viet Truong Hong, I-Tung Chen, Bunlung Nuangsaeng, Orasa Suthienkul, Chu Fang Lo, Timothy W. Flegel, Kallaya Sritunyalucksana and Siripong Thitamadee (email, Centex Shrimp, Faculty of Science, Mahidol University, RamaVI Road, Bangkok, Thailand).  In Press, Accepted Manuscript.  Available Online on March 28, 2014.

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