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July 1, 2013


Double Stranded RNA to Control IMNV and LSNV


From Abstract: Development of specific and effective methods for controlling shrimp viral diseases is essential to lower the risk of severe economic losses. RNA interference (RNAi) is a specific and effective approach for inhibiting viral replication by introducing double-stranded (ds)RNA to interfere with viral mRNA.  Here, long sequences of dsRNA were prepared to target two serious shrimp viruses, infectious myonecrosis virus (IMNV) and Laem-Singh virus (LSNV).  IMNV infection has caused high mortalities of Penaeus vannamei in Brazil and Indonesia, and LSNV is a possible causative agent for P. monodon slow growth syndrome (MSGS) in Thai P. monodon.


During 1-month observation after IMNV challenge, P. vannamei receiving viral-specific dsRNA exhibited considerably higher survival rate (63%) than those receiving non-specific dsRNA (17%).  In addition, viral-specific dsRNA could be administered orally in P. monodon for effective reduction of LSNV.  The degree of LSNV inhibition appeared to be dsRNA-dose dependent.  Sixty percent LSNV-free shrimp was observed in the group fed with 12 mg dsRNA/kg feed, and 30% viral-free shrimp was found under treatment with 6 mg dsRNA/kg feed.  For the group that did not receive dsRNA (negative control), all tested shrimp were infected by LSNV.  The average size of the negative-control shrimp was significantly smaller relative to those shrimp fed with specific dsRNA.  This study demonstrates effective use of dsRNA for the control of viral-related shrimp diseases.


Source: The Thumb Drive Containing the Abstracts from Aquaculture 2013 (February 21-25, 2013, Nashville, Tennessee, USA).  Information: John Cooksey, World Aquaculture Conference Management, P.O. Box 2302, Valley Center, California 92082 USA (phone 1-760-751-5005, fax 1-760-751-5003, email  Using Double-Stranded RNA for the Control of Shrimp Diseases IMNV and LSNV.  Vanvimon Saksmerprome (Center of Excellence for Shrimp Molecular Biology and Biotechnology, Faculty of Science, Mahidol University, Rama VI Rd., Bangkok 10400, Thailand) Kallaya Sritunyalucksana, Thitipon Thammasorn, Sarocha Jitrakorn, Nutcharee Songsakul, Chantra Saelim, Chompunut Kirdmusik, Theerapong Chutipongraksa, Timothy W. Flegel and Boonsirm Withyachumnarnkul.  February 2013.


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