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March 29, 2016

Thailand

Research—Visual Detection of AHPND Bacteria

 

This study was published in its entirety on-line by PLOS ONE, a peer-reviewed, open access (free) scientific journal published by the Public Library of Science (PLOS) under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.  Operating under a pay-to-publish model, all submissions go through a pre-publication review by a member of the board of academic editors, who can elect to seek an opinion from an external reviewer.  As of 2014, PLOS ONE was the world’s largest journal by number of papers published.

 

From Abstract (Click on the link in the Source below for a free, full copy of the paper.)

 

After AHPND was characterized, a major focus of the AHPND control strategy was to monitor broodstock shrimp and post larvae for freedom from AHPND by nucleic acid amplification methods, most of which required use of expensive and sophisticated equipment not readily available in a shrimp farm setting.  In this study, researchers describe a simpler but equally sensitive approach for detection of AHPND based on loop-mediated isothermal amplification (LAMP) combined with unaided visual reading of positive amplification products using a DNA-functionalized, ssDNA-labled nanogold probe (AuNP).

 

Source: PLOS ONE.  Sensitive Visual Detection of AHPND Bacteria Using Loop-Mediated Isothermal Amplification Combined with DNA-Functionalized Gold Nanoparticles as Probes.  Narong Arunrut, Jantana Kampeera, Sarawut Sirithammajak, Piyachat Sanguanrut, Porranee Proespraiwong, Rungkarn Suebsing, Wansika Kiatpathomchai (Email: wansika@biotec.or.th, Bioengineering and Sensing Technology Laboratory, BIOTEC, National Science and Technology Development Agency (NSTDA), 113 Thailand Science Park, Phahonyothin Road, Khlong Nueng, Khlong Luang, Pathum Thani 12120, Thailand).  March 22, 2016.

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